The present study was conducted on 36 Wistar male rats which were equally divided in three groups (n=12) viz. T1 group (treated with Nano silver gel), T2 group (treated with Platelet Rich Plasma (PRP)) and T3 group (treated with Povidone-iodine) which served as control group. Treatment was done at first 7 days from wound creation and tissue was collected on 7th, 14th and 21st days from wound creation, and exhibited the effect of topical application of silver nanoparticle and PRP in full thickness excisional wound in diabetic rats. The aim of the research work was to compare and evaluate the wound healing potency of Nano silver gel and PRP on wound healing process after its topical application in full thickness excisional wound in diabetic rat models. T2 showed significantly (p<0.05) better wound healing in terms of reduction in wound size, % wound contraction and catalase enzyme activity while numerically in terms of degree of inflammation, type and quantity of exudate, appearance and colour of granulation tissue, on days 7, 14 and 21 followed by T3 as compared to T1. Better catalase enzyme activity is indicative of increment in collagen deposition in reparative wounds which in turn is indicative of better wound healing. Application of nano silver gel and PRP enhance the wound healing process in diabetic wounds as compared to povidone-iodine treated wounds.