Lime (Citrus aurantifolia Swingle) is one of the important commercial fruit cultivated in Jammu region. The experiment was conducted to study variability among acid lime genotypes. A survey was undertaken during 2017 to 2019 in five districts of Jammu province. The study comprised of characterization and evaluation of lime genotypes that were identified in different areas. Seventy acid lime genotypes from different areas were selected. The results revealed that maximum tree height (4.2 m) was recorded in genotype JMU-Nag(66). Most of the genotypes were obloid in tree shape with spreading tree growth habit, dense branching habits and narrow branch angle. Among fruit characters maximum fruit weight (56.26 g), fruit diameter (49.57 mm), fruit length (55.70 mm) and total yield (160.28 Kg/tree) was recorded in genotype JMU-Nag(70) whereas width of epicarp at equatorial area (2.50 mm) was recorded maximum in JMU-Log(9). Cluster dendrogram grouped seventy lime genotypes under two main clusters A and B representing the intensity of diversity and closeness among them. Cluster-A had four genotypes and cluster B is further divided into nine sub clusters comprising of sixty-six genotypes. As cluster I comprised of only four genotypes and formed the separate and most distinctive cluster representing JMU-Pana(41), JMU-Jib(36), JMU-Nag(65), JMU-Nag(70) as the most diverse genotypes and can be used for further breeding programme.