Organic waste from animals and plants can be processed into Biourine. Biourine is useful for plants as a liquid organic fertilizer. Processing livestock waste from urine into biourine aims to avoid environmental pollution and help plant fertility as liquid organic fertilizer. This study aims to determine the benefits of organic waste from animals and plants to manufacture biourine as liquid organic fertilizer. This study used cow rumen from animals and tomato waste from plants, using a randomized block design with five treatments and eight replications. namely A-: Cow urine without addition, A+: Cow urine + EM4, B: Cow urine + 100% animal IMO, C: Cow urine + 100% vegetable IMO, D: Cow urine + 50% animal IMO + 50% vegetable IMO. Parameters observed were total microbes, pH, macro-nutrients Carbon (C), Phosphorus (P), Nitrogen (N), and Potassium (K), as well as micro-nutrients iron (Fe) and Copper (Cu). The results showed that total microbes, pH, macronutrients, the five treatments showed different results (P<0.05), and while micronutrients showed no different results (p<0.05), the conclusion of organic waste originating from animals and vegetables can be used as basic ingredients for biourine production as a liquid organic fertilizer that is useful for food crops.