The versatility use of polyacrylamide has gained momentum in the recent past with its targeted use as a fertilizer when combined with urea through polymerization under specific conditions to develop ureapolyacrylamide (UPAM). After polymerization, ureapolyacrylamide of can be developed into four various grades with respective nitrogen (N) and carbon (C) concentrations (UPAM-1: C-35.35%&N-32.35%; UPAM-2: 27.67&39.40%; UPAM-3: 25.11&41.61; UPAM-4: 23.83&42.71). The experiment laid out in a randomized block design with three replication and ten treatments consisting of the following T1-absolute control, T2-UPAM-1, T3-UPAM-2, T4-UPAM-3, T5-UPAM-4, T6-UPAM-1, T7-UPAM-2, T8-UPAM-3, T9-UPAM-4 and T10-neem coated urea (NCU), here T2 to T5 treatments are two split applications and T6 to T10 treatments are three split applications. The objective of this study was to assess the effect of various grades of UPAM application on soil microbial enumeration, soil microbial biomass, and enzymatic activity in rice and maize rhizosphere. The study showed dominant soil microbial enumeration, enzymatic activity, and microbial biomass under two (T3) and three (T7) split applications of UPAM-2 grade polymeric N treated plots in maize and rice crops. There is a significant impact on dehydrogenase and urease enzyme activity and a non-significant impact on microbial biomass carbon, soil alkaline phosphatase activity, and soil microbial enumeration in both crops. The results also showed that the application of various grades UPAM along with SSP and MOP indicated a positive impact on soil biology, and no negative impact was recorded in both crops under two consecutive crops.