The use of neonicotinoides insecticides, particularly acetamiprid, in agriculture is increasing day by day to increase crop yields, but their excessive use has reduced beneficial soil fauna and flora, which directly or indirectly affects soil health. Crop yield is inversely proportional to invertebrate flora, with earthworms being the most affected organisms by pesticides. During pest management programmes in agricultural fields, through direct application as well as foliar wash-offs or drift, large amounts of acetamiprid active ingredients are typically deposited on the soil. Studies on the impacts of neonicotinoids pesticides have primarily focused on acetamiprid's effects on earthworm’s morphological parameters as well as behaviour. Acetamiprid LC50 values derived using the Probit analytical method were 0.165g/cm2, which is the pesticide concentration that kills 50% of earthworms. As the concentration of acetamiprid rises, so do the negative effects on earthworms, with bulging of the anterior end, body constriction, preclitellar swelling, clitellum bulging, tapered end swollen, oozing out of coelomic fluid, loosened segments, formation of a knot-like structure at the anterior end, cuticle rupture, and desegmentation among the changes. Furthermore, earthworms demonstrated dark coloration, leaking out of hemocoel, perforated cuticle, and epidermal peeling at an acetamiprid dosage of 0.240g/cm2.