Bananas are commodities that are easily cultivated and developed in Indonesia. Optimizing plant growth is carried out through organic fertilizer and Arbuscular Mycorrhizal Fungi (AMF). This research aims to get the right type of organic fertilizer and AMF and understand whether there is an interaction between organic fertilizer and AMF dose to increase the growth of the banana plant Raja Bulu of tissue culture. The research was located in the experimental field of the Faculty of Agriculture, University of Sebelas Maret, Karanganyar. The research design used was a factorial, completely randomized design with a combination of 2 factors. The first factor is the type of organic fertilizers goat manure, cow manure, vermicompost, and compost. The second factor is the dose of AMF 0 g, 5 g, 10 g, and 15 g. The data obtained were tested statistically by statistical analysis with ANCOVA based on the F test level of 5%. If it had a real effect, further tests were carried out using DMRT with a 5% level. The variables observed were plant height, number of leaves, leaf width, leaf length, mycorrhizal infection, and stem diameter. Results showed an interaction between the types of organic fertilizer and the doses of AMF against the diameter of the stem of the plant of banana Raja Bulu. Goat manure can increase the growth of banana Raja Bulu better than the treatment of compost fertilizer. AMF dosage treatment of 5 g to 15 g per plant has not increased the growth of the banana Raja Bulu.