Among the sustainable and more respectful alternative strategies for man and his environment, arbuscular mycorrhizal fungi are used to limit or reduce fertilizers constitutes a problem in terms of cost and damage to the environment and soil. This study evaluated the effects of chemical fertilization and native mycorrhizal fungi collected from palm grove rhizospheres on the growth of wheat (Triticum durum Desf). NPK fertilizers were applied at four levels (0, 25, 50, and 100%) with or without native AMF inoculation. Results of all studied parameters showed that 25% and 50% chemical fertilizer combined with AM fungi gave the best growth improvement in wheat plants compared to inoculation with AMF alone. The other treatments with 100% chemical fertilizer combined with native AMF showed a decreased effect and mycorrhizal colonization is reduced by about 40%. This suggests that the combined application of low levels of chemical fertilizer and AMF can increase wheat growth and yield more efficiently than the application of either alone. On the other hand, it will preserve the environment and soil health, so the use of native AMF has an interesting economic and environmental advantage.