This study aimed to evaluate the effects of age, gender and physiological state on liver parameters. 218 samples of clinical healthy crossbreed sheep raised at Haidar farm of Ain Gasma, Tiaret province, used to measure the plasma activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma glutamyl transferase (GGT), alkaline phosphatase (ALP) total bilirubin (TBIL), direct bilirubin (DBIL), albumin (ALB), total protein (TP). The research highlights the significant impact of age on all the parameters, but no significant difference in terms of blood GGT, ALB and TBIL levels among male groups was noticed, while gender was considered as secondary factor influencing blood ALP, ALT, TP, DBIL and TBIL levels. In addition, the physiological stage has an impact on GGT and ALB parameters, In conclusion, to interprate the Hepatic metabolism, we need to take into consisderation, the physiological status, the gender and the age of the sheep.