Honey bees occupy a prime position among all the pollinating agents because they can be managed in artificial nests, i.e. hives and can be placed in desired numbers. In Himachal Pradesh, the beekeepers migrate their colonies to other places during winters due to scarcity of bee flora. To keep the honeybee colonies strong during dearth periods different pollen substitutes and supplement need to be evaluated as a part of good management practice for beekeeping. Various ingredients were used such as defatted soya-flour, wheat flour, yeast, gram powder, skimmed milk various grain flour, sugar, honey etc. These mixtures were used in different ratios to evaluate their influence on colony parameters of A. mellifera L. The colonies were fed on various pollen substitute and supplement diets by frame feeding method at 14 days interval during rainy-autumn and spring-summer season. All the pollen substitutes were accepted by A. mellifera colonies and the consumption rate within 14 days varied in the range of 80.62% to 97.65% during both the seasons. A significant increase in brood area (1.61 times), honey stores (3.41 times during rainy-autumn, 2.87 during spring–summer) and pollen stores (1.77 times during rainy-autumn, 1.43 during spring–summer) was observed over control. On the basis of these studies, it was suggested that various pollen substitutes showed positive effect on colony parameters and honey stores of A. mellifera as compared to control.