The article presents the results of studying the effect of humic and bacterial preparations, complex liquid micronutrients when treating spring barley seeds on the microbial community of gray forest soils of Ryazan region of the Russian Federation. Studies of microorganisms of various ecological and trophic groups of gray forest medium loamy soil of the studied crop rotation using conventional methods showed that preparations and their complexes used in pre-sowing seed treatment influenced the number of soil microorganisms and their biological activity. It was found that the pre-sowing treatment of seeds with bacterial preparation Rhizoagrin made it possible to sharply increase the content of Firmicutes bacteria in the soil, participating in the decomposition of organic residues. The main part of the microbiome in the variants with humic preparation Ekorost consisted of Actinobacteria bacteria, the major component of which was bacteria of the Micrococcaceae family, which caused the transfer of hard-to-reach nutrients, such as urea, into an ammonia form more accessible to plants. A large proportion of diazotrophic Proteobacteria bacteria was also noted.