Pearl grass is a plant that can be used as a medicine for various diseases, such as cancer, hepatitis, diabetes, wounds, eye pain, fever, and stomach. Pearl grass contains several chemical compounds, and of them are ursolic acid and oleanolic acid. Using chicken manure and arbuscular mycorrhizal has the potential to be a cultivation technology to determine ursolic acid levels in the pearl grass. The research was carried out in Jumantonso, Karanganyar, Central Java. The research was conducted from December 2019-February 2020. The study used a completely randomized design (CRD) with 2 factors. The first factor is chicken manure at a dosage of 0, 7.5, 15 tons/ha. The second factor was arbuscular mycorrhizal at the dosage of 0, 5, 10, 15 g/plant. The treatment combination consisted of 5 replications. The variables observed were plant height, number of branches, plant crown diameter, root length, plant root weight, fresh plant weight, plant dry weight, and ursolic acid content. The results showed that the application of chicken manure 7.5 tons/ha was able to affect all parameters except for the length and weight of pearl grassroots. The various doses of arbuscular mycorrhizal did not affect all growth and yield parameters of pearl grass. Application of chicken manure and arbuscular mycorrhizae resulted in high levels of ursolic acid in most treatment doses. The application of chicken manure and arbuscular mycorrhizae increased the growth and yield of pearl grass but did not provide interaction on the growth and yield of pearl grass.