To determine the impact of fertilizer (Fosfactyl) on the growth of durum wheat during water stress. Our research has indicated the importance of chemical fertilizer (Fosfatyl) in improving durum wheat's behavior by directly impacting abscisic acid (ABA) expression. Most plant tolerance to water stress is attributed to a phytohormone. The amount of abscisic acid is increased by plants when faced with adverse stress. Plant resistance increases under long-term adversity stress due to the accumulation of abscisic acid (ABA) in plants. Water stress treatment affects the response variability of three durum wheat genotypes (Simeto, Hedba-3, and GTA dur) differently. By analyzing multiple physiological and biochemical parameters, the tolerance strategy adopted by each variety was characterized. Fosfactyl under water stress resulted in a change in seedling physiology, as well as an accumulation of proline, proteins, and sugars, which led to an increase in antioxidant enzyme activity. The experimental work has demonstrated that the response of durum wheat varies from variety to variety and is based on the fertilizer applied. The examination suggests that certain parameters are reliable indicators of tolerance and can handle the pressure of water stress.