This study investigated the variation in nutrient content in grape petioles and berries throughout the growing season. The results showed that the mean nitrogen (N) content in petioles ranged from 0.67% to 1.37% and exhibited a decline over the sampling period. Anab-e-Shahi cultivar had higher N levels compared to Perlette. Petiole N showed a positive correlation with phosphorus (P) and potassium (K). Similar periodic fluctuations in petiole N content were observed in other grape cultivars. The mean P content varied from 0.12% to 0.34% and showed a non-significant decrease during early growth stages and a subsequent increase. Petiole P exhibited positive correlations with K and N. The K content increased initially and then decreased throughout the season, with Perlette having higher levels than Anab-e-Shahi. Petiole K showed positive correlations with N and P. The calcium (Ca) content increased gradually, while magnesium (Mg) showed an overall increase from D1 to D9. Boron (B) increased initially and then decreased towards the end of the season. While comparing nutrient status in both leaves and fruits from D6 to D8 macronutrients show maximum flow from source to sink. In case of Nitrogen D6 showed maximum decrease in N in leaves and increase in fruits which means maximum source to sink is in D6 and majority of macro nutrients show similar behavior, while as in case of micronutrients initial stages D5 onwards source to sink gets decreased hence micronutrient sprays should be recommended at initial stages hence nutrient sprays are recommended during these stages for maximum uptake of nutrients from leaves to berries for maximum nutrient fortification in berries. These findings emphasize the importance of considering specific reference levels for each phenological stage and highlight the dynamic nature of nutrient content in grapevine growth and development.