We employed the classical microbiological methodology to isolate endophytic fungi inhabiting the rhizosphere of maize (Zea mays L.). Identification of these endophytic fungi was carried out using ribosomal DNA sequencing, aiming to uncover distinct distribution patterns of these organisms that persist asymptomatically within the healthy root tissues of maize plants. The dominant fungal species associated with the roots of the maize hybrid PHM-1 were Aspergillus niger, Aspergillus terreus, Cladosporium cladosporidies, Penicillium oxalicum, and Penicillium raperi. To assess their potential impact, an in vitro assay was conducted to evaluate these isolates against Rhizoctonia solani f. sp. sasakii, the causal agent of banded leaf and sheath blight (BLSB). This disease is particularly prevalent in tropical regions worldwide and poses a significant threat to maize cultivation. Notably, the Aspergillus and Penicillium complexes found in the maize rhizosphere exhibited promising efficacy in inhibiting this soil-borne disease affecting maize plants.