To assess the incidence and severity of citrus canker disease in different districts of Jammu region, a comprehensive survey was conducted across five districts viz. Samba, Jammu, Udhampur, Kathua and Reasi. The results revealed regional and seasonal variations with Samba district of the area exhibited the highest mean Percent Disease Index (PDI) of 50.81% during August-September period which gradually decreased across the months. The pathogen of citrus canker was isolated, identified and confirmed through pathogenicity testing. In vitro evaluation revealed that Bacillus subtilis was the most effective bio-control agent, producing an 18 mm inhibition zone, while among botanicals, neem extract showed the highest efficacy with a 15 mm inhibition zone and copper oxychloride was identified as the most potent fungicide, with a 25 mm inhibition zone, while streptomycin sulfate exhibited the strongest antibacterial activity among antibiotics, achieving a 22 mm inhibition zone. These findings underscore the importance of integrated disease management strategies, combining biological, chemical and botanical approaches to effectively control citrus canker and reduce its impact on citrus production in the region.