This research were conducted to study the effect of different photoperiod in chrysanthemum cv. Autumn white and Diana orange for the period of 1st October 2021 to 20th March 2023 in climate controlled greenhouse at Centre for Protected Cultivation Technology (CPCT), IARI, New Delhi. Two spray Chrysanthemum varieties such as Autumn white and Diana Orange was exposed to 3 various day length extension treatments under semi climate controlled green house with use of Incandescent lamps for 10, 15 and 20 days duration and each 4 replications. Various observation in respect to growth and flowering were recorded at different intervals (0,15, 30,45 and 60 days). Vegetative growth parameters like plant height (95.79 cm), stem diameter (6.56 mm), inter nodal length (4.41cm), total number of leaves (39), leaf area (262.52 cm2), leaf area index(3.22), leaf fresh (24.91g) and dry weight(2.61 g), stem fresh (21.88g) and dry weight (3.23g) were measured and maximum under 20 days extended day length at 60 days after planting. Flowering parameters like bud diameter (16.78 mm), flower diameter (87.68 mm), time taken for flower bud induction(89.3days), time taken for flower opening (100.7 days), flower fresh(13.57g) and dry weight (1.59g) were taken at different blooming time intervals and highest were found in Autumn white at 60 days after planting for 20 days. Among two different varieties Autumn white showed significant difference than Diana orange.