This study was undertaken to evaluate the effect of gamma irradiation on morphological, yield and biochemical attributes of sweet potato [Ipomoea batatas (L.) Lam.] cv. ST-14. Cuttings of sweet potato vine were irradiated with gamma rays (15, 30, 45, 60, 75, and 90 Gy) and non-irradiated cuttings were chosen as controls. After irradiation, cuttings were planted in replication in a well-prepared field at 30 x 30 cm spacing. Result revealed that average days to 50 (%) sprouting was significantly delayed with increasing doses of gamma irradiation. Among the gamma irradiation earliest day of 50 (%) sprouting was recorded in 15 Gy (12.49 days), whereas the delayed sprouting was recorded at 45 Gy. The maximum sprouting per cent, survival per cent and vine length was recorded in control followed by 15 Gy. Exposed to higher dose of gamma irradiation tends to decease in sprouting per cent, survival per cent and vine length of sweet potato. The LD50 value occurred at 45 Gy gamma irradiation level. The results indicated that gamma irradiation treatment was adversely affected vegetative parameters, such as vine length, number of leaves, leaf length, leaf width, and average fresh weight above ground level were recorded in irradiated plants over the control. TSS, starch content, sugar percentage, and carotenoids content were unaffected by irradiation treatment and were found to be non-significant in both generations. In conclusion, our findings indicated that gamma irradiation had a significant impact on vegetative and yield characteristics but had no effect on biochemical properties.