Journal ID : AMA-20-12-2022-11903
[This article belongs to Volume - 53, Issue - 12]
Total View : 451

Title : Studies on effectiveness, efficiency of gamma rays, EMS and their combination in M2 generation of Him Palam Hara Soya 1 variety of soybean [Glycine max (L.) Merrill]

Abstract :

Genetic improvement of soybean is needed to increase its production. Mutation breeding is the highly useful and vital technology for soybean improvement. Selection of effective and efficient mutagens are very essential to recover high frequency of desirable mutants. In order to provide genetic diversity and resources for identifying important genes, physical treatment of gamma rays, chemical treatment of ethyl methane sulfonate (EMS) and their combination dose was used to mutagenize soybean variety Him Palam Hara Soya 1 (Himso-1685). The seeds were treated with physical mutagens i.e. gamma rays at five different doses (100 Gy, 200 Gy, 300 Gy, 400 Gy and 500 Gy) at BARC, Trombay, Maharashtra, EMS at 0.5%, 1.0 % and their combination was in the Department of Genetics and Plant Breeding, CSK HPKV, Palampur, H.P during year 2019. A total of 256 M1 individuals were produced from 6,800 treated seeds. Five types of mutants based on phenotypic appearance were identified viz., dwarf plants in comparison to tall plants of control, white flower as compared to purple flower, of control, brown fawn colour on pods as compared to gray fawn colour on pods of control, reduced seed size as compared to bold seed size of control and complete resistant plants prevailing white flowers as compared to purple colour of control were observed in the present study. Gamma rays were found to be more effective as compared to chemical and combined dose to induce vital mutations in variety Himso-1685. Higher the dose or concentration, more reduction in the expressivity of the traits was observed. Beneficial traits from these mutants can be exploited for future soybean breeding programs. This variety Himso-1685 can also be used for discovering novel mutant alleles and functional gene expression analysis using reverse genetics tools such as Tilling.

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