The laboratory experiment on “Studies on effect of different soil types and moisture regimes on seed hydration value of various soybean varieties” was conducted during the season 2020-21 in CIC laboratory of Soil Science and Agricultural Chemistry Department, College of Agriculture, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani, to find out the seed hydration value and germination in different soybean varieties at different soil moistures in three types of soil. This study was carried out in petri plates with four soybean varieties JS-335, MAUS-71, MAUS-158 and MAUS-162 at four soil moisture levels of 50, 75, 100 and 125 percent field capacities in Vertisols, Entisols and Inceptisols soils. The treatments were replicated two times in FCRD design and observations were taken at regular time interval of 6 hours up to 96 hours from sowing. The results obtained from investigation revealed that the germination parameters and seed hydration value for different soybean varieties were clearly influenced by different soil moistures in different soil types. Amongst all varieties, variety MAUS-162 showed highest germination at different soil moistures and lowest was noted in variety JS-335. In vertisols and Inceptisols, the highest seed hydration value was reported in variety MAUS-162 with earliest germination among all varieties in all soil types. Better germination for soybean varieties were observed at soil moisture of 75 and 100 percent field capacities. Vertisols and Inceptisols showed early and better germination for soybean seeds. Among all soybean varieties, MAUS-158 and MAUS-71 showed lower seed hydration value so can be suitable for rainfed cultivation.