The present research study was undertaken to evaluate compatibility and comparative efficacy of different microbial bio-agents with botanicals as well as safer conventional fungicides against anthracnose in mango. Among fungal bio-agents viz. T. harzianum and T. virens observed compatible with azoxystrobin fungicide only, while bacterial bio-agents viz. Bacillus subtilis and P. fluorescens were found compatible with trifloxystrobin + tebuconazole. The present study also describes very effective approach of integrated disease management. However, three consecutive prophylactic spray was done with 10 days of pre-harvest intervals (PHIs) starting with mid of July for consecutive two crop seasons with in vitro most effective combi-biofungicides in which trifloxystrobin + tebuconazole (0.03%) + Bacillus subtilis (5%) was assayed highly efficacious with mean % disease index of 15.1 and 12.0 per cent with mean disease control 77.9 and 80.9 per cent at two different experiment location, respectively. Additionally, dip treatment of mango fruits after harvest in combined treatment consists of combi-biofungicides, trifloxystrobin + tebuconazole (0.03%) + Bacillus subtilis (5%) for the period of 5 minutes proven highly effective with 93.4 % Disease Reduction Index (DRI) which is followed by other combined treatment of trifloxystrobin + tebuconazole (0.03%) + P. fluorescens (5%), difenoconazole (0.012%) + P. fluorescens (5%) and with mean DRI 91.7 and 89.9 per cent, respectively. Overall, this research study underscores the importance of using efficacious combi-biofungicides in integrated manner which could be eco-friendly alternative over synthetic fungicides usage against fruit disease ensuring successful disease management in mango.