Among fifteen populations investigated, two were identified as belonging to the Steinernema genus. Within this genus, the majority of populations exhibited infective juveniles (IJs) with horn-like cephalic papillae. In the sandy and sandy loam soils of southern Haryana, Steinernema was more prevalent compared to Heterorhabditis. Two new species were distinguished based on specific morphological characteristics. The first species, S. numeropapillatum n. sp., featured IJs with two horn-like structures on their head regions. These IJs had an average body length of 636.7 μm, a tail length of 57 μm, D% of 45.4, and E% of 85. First-generation males had spicules measuring 71.7 μm, a gubernaculum length of 41.2 μm, SW% of 150, and GS% of 57.6. Females lacked epiptygma and had non-mucronate tails. The second species, S. jujubum n. sp., was characterized by infective juveniles with a body length of 700 μm, an excretory pore at 50.7 μm, and a lateral field with eight ridges, four of which were raised while the outer two on each side were low. First-generation females had a nearly median vulva with epiptygma but lacked a mucronate tail. Males had large spicules (73.7 μm), tails without mucrons, and a rounded spicule manubrium with continuous shaft, equal ribs, and blunt tips. The gubernaculum was ventrally curved without a proximal hook and had a short neck. Copulatory papillae numbered 27, consisting of 13 pairs and a single large midventral precloacal papilla. This new species was grouped with other Steinernema species bearing "horn-like structures."