The present study was aimed for standardization of seed germination and assessment of quality traits of Moringa oleifera seedlings grown in different substrates under dryland semi-arid conditions. PCA using the twenty seven traits of eighteen treatments of M. oleifera indicated that 90.16 % variability was accounted for the first four principal components (PCs) with eigen values ≥1 (Fig 2-4). The 1st principal component (PC1) had an eigen value of 12.00 and explained 44.43 % of the total variation. The second component (PC2) explained 27.30% of the total variance with an eigen value of 7.37 and explained 71.73 % of the total variation. G6DAS and G8DAS with G6DAS, G8DAS with G6DAS, G12DAS and G14DAS with G10DAS, G14DAS with G12DAS, SSNS45DAS and LL45DAS with SSEW45DAS, LL45DAS with SSNS45DAS, P and K with DM, K with P, Mg and DPPH with Ca, DPPH with Mg were significantly and highly correlated r = +1.0. High magnitude and significant correlation were found between St45DAS and G10DAS, St45DAS and Se45DAS, ViC with SSEW45DAS, SSNS45DAS & LL45DAS, TF and ViC, TP & TF with Ca & Mg, DPPH with TP & TF, CUPRAC and FRAP r = +0.9. The inter cluster distance (D) ranged from 770.33 to 1590.58. The intra cluster distance (D) ranged from 142.25 to 957.11. cluster I had maximum mean value for sixteen traits viz. G4DAS, G6DAS, G8DAS, G10DAS, G12DAS, G14DAS, Se45DAS, St45DAS, LNS45DAS, Mo and Fe and minimum mean value for LNL45DAS, Mo, P, K and TF while moderate mean value for traits SSEW45DAS, SSNS45DAS, LL45DAS, ViC, Ca, Mg, TP, FRAP, CUPRAC and DPPH. The maximum germination was recorded in cluster I.