A field study was carried out to investigate the soil biological properties and yield of sweet corn (Zea mays L. saccharata) as influenced by different sources of nitrogen in typic haplusteps of Rajasthan during kharif, 2019 at the Instructional Farm of the Rajasthan College of Agriculture, Udaipur with twelve treatments i.e. 100% RDN through urea, 75% RDN through urea + 25% N – FYM, 50% RDN through urea + 50% N – FYM, 75% RDN through urea + 25% N – VC, 50% RDN through urea + 50% N – VC, 50% RDN through urea + 25% N – FYM + 25% N – VC, 125% RDN through urea, 100% RDN through urea + 25% N – FYM, 75% RDN through urea + 50% N – FYM, 100% RDN through urea + 25% N – VC, 75% RDN through urea + 50% N – VC, 75% RDN through urea + 25% N – FYM + 25% N – VC. . Experiment was laid out in randomized block design with three replications. Results revealed that application of 125% RDN through urea recorded significantly higher green cob yield, green stover yield and green fodder yield. However, soil biological properties like microbial population and enzymatic activities were highest with application of 75% RDN through urea + 50% N – FYM. The present findings indicated that 125% RDN through urea recorded higher yields, but to sustain the soil health 75% RDN through urea and 50% RDN through FYM have been recommended.