Si is also known as a multifunctional element that significantly increases plant tolerance and enhances resistance against insect and pests. Okra (Abelmoschus esculentus L.), commonly known as lady's fingers or gumbo, faces susceptibility to diverse pests, including root-knot nematode (Meloidogyne incognita), a prominent plant pathogen globally within the Malvaceae family. Quantitative PCR (qPCR) is one of the most common and accurate methods of gene expression analysis. Reference genes are commonly used as the endogenous normalisation measure for the relative quantification of target genes. Gene expression study using RT-PCR revealed upregulation of PAL, CAD, and CHS in disease conditions, while Lsi 1 and Lsi 2 were upregulated in silicic acid treatment. Silicon amendment in okra roots elicits a priming defence response against Root knot nematodes. Si-induced resistance correlates with the activation of PAL, CAD, and CHS genes during disease conditions following nematode attack.