In Algeria, although a control plan (screening and slaughter) has been in place since 1995, the evolution of animal and human brucellosis remains variable from year to year. The present investigation is carried out with the aim of determining the prevalence as well as the insufficiencies of the applied control plan. For this purpose, 100 blood samples of cattle, distributed over 19 visits, were taken during the month of April 2020 in the slaughterhouse of Sidi-Bel-Abbès. Data related to age, identification, breed, type of slaughter, pregnancy, and the presence of metritis were also collected. In addition, 10 sets of volunteer professionals from the same establishment had their socio-epidemiological information collected. Thus, all sera were analyzed by the buffered antigen test (BAT) and the Wright test. The results obtained show, on the one hand, a 3% positivity to the EAT and compliment fixation (CF) in slaughtered cattle and 0% in professionals, and, on the other hand, an overall rate of 10% daily occurrence of brucella cattle within the slaughterhouse. In addition, 2.80% of the cattle slaughtered in the framework of sanitation are positive. While no difference in prevalence related to breed, identification status, or age is recorded, In fact, the uncontrolled movement of animals was found to be a limiting factor in the effectiveness of brucellosis control. These results encourage the application of strict prophylactic and preventive measures against brucellosis and the renovation of slaughterhouses and killings in Algeria.