A study on the “Self Sufficiency in Fish Production through Biofloc farming for Doubling the Small Holder Farmers’ Income of Manipur” shows that the initial fixed cost incurred in Biofloc farming was found to be Rs. 40000 including the installation charges while the expenditure on variable cost were approximately estimated as Rs. 104200. In totality, a farmer in the study area incurs or spent an amount of Rs. 144200 for undergoing Biofloc farming. Usually the farmers produce 2 crops in a year and each crop or I-crop can harvest upto 600 kg with a gross cost of Rs. 180000 and a net return of Rs. 35800. Thus a Biofloc farmer harvested 1200 kg with an annual net return of Rs. 71600 from the 2 crops on an average. The return over investment for three (3) years for an unit Biofloc farmer shows the increasing return over the period and it was found to be 16.5%, 67% and 71.10% for I; II and III year respectively. Institutional mechanism like low access to financial credit; high cost of fingerlings and their availability, reliability & survivability; high cost of fish feed, lack of extra rearing chambers/ponds during the pre-culture period; lack of tip-top scientific skill and limited fish species for Biofloc farming are ranked as the production problem and issues like price spread; low demand and lack of Govt. policy in the marketing aspects. Proper institutional approach or mechanism of backward linkages for the helpless or marginal farmers in order to invest in such high capital enterprise like Biofloc; better awareness and skill oriented outreached activities from the professional end can grow and develop Biofloc farming in the district.