Journal ID : AMA-21-10-2021-10808
[This article belongs to Volume - 52, Issue - 02]
Total View : 334

Title : Rooting and Adaptation of Solanum tuberosum L. under Ex Vitro Conditions after Exposure to Different Sucrose Concentrations in the In Vitro Nutrient Medium

Abstract :

The composition of nutrient media intended for the formation of morphogenic callus in tissue culture is a key element of the intermediate link in the production of plants in vitro. The six potato varieties of the Ukrainian breeding of different ripeness groups early-ripening, mediumearly, medium-ripening were used as the research object. Fragments of the apical meristem of plant organs were selected for the introduction of in vitro in the culture. Parts of stems with axillary buds were used to produce callus tissue. The Murashige and Skoog media (Murashige and Skoog, 1962) with sucrose content modifications of 30 and 60% were used to induce callus genesis and growth of the resulting callus. The control version of the medium contained 10% sucrose. The resulting material was planted in the open ground on the experimental plots of Sumy National Agrarian University. The results of the studies indicate a generally positive, but uneven effect on the rooting nature of test tube plants of the varieties used. Individual variants of potato cultivars of different ripeness groups grown on a medium of both 10 and 30% and 60% sucrose content are considered to be promising for further reproduction and use in the breeding process.

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