Tomato (Solanum esculentum Mill) is an important vegetable crop and grown all around the world. It is one of the most important host for plant parasitic nematodes, mainly root-knot nematode (Meloidogyne javanica). Experiments was conducted to test the efficacy of botanicals (viz., garlic, parthenium, aak, datura, marigold and neem) against root-knot nematode in laboratory (0.5, 1, 2, 5 and 10 per cent concentration) as well as in pot and field condition (10 per cent concentration), PPO and PAL and Phenol were also estimated in tomato roots infected with root-knot nematode (M. javanica). The experimental results showed that all the botanicals significantly reduced the per cent hatched juveniles and increased the per cent mortality of juveniles, increased the level of PPO, PAL and phenol in tomato roots, increased the plant growth parameter and reduced the nematode reproduction as compared to untreated check. Among the tested botanicals neem leaves extract was found most effective treatment with minimum per cent hatched juveniles and maximum per cent mortality of juveniles @ 10 per cent concentration after 72 hrs followed by parthenium and garlic extract. Similarly, neem leaves extract @ 10 per cent concentration was found to be the best treatment to enhance PPO, PAL and phenol activity, plant growth parameter as well as in reducing nematode population in pot as well as field condition.