Mungbean yellow mosaic disease is caused by the Mungbean yellow mosaic virus or MYMV that causes damage to Mungbean plants. The white fly-Bemesia tabaci is the insect vector that transmits the virus. Overall, Mungbean yellow mosaic disease is one of the main destructive diseases of Mungbean species and varieties. The current study was conducted to screen 36 genotypes of mungbean germplasm against mungbean yellow mosaic virus disease under natural field conditions. In the field, all the genotypes were subjected to natural infection by infectious whiteflies. The maximum and minimum disease incidences (%) were observed in GP-276 (18.08%) and BRM-8-1 (0.47%) respectively at 55 DAS. The maximum and minimum disease severities (%) were observed in GP-276 (56.3%) and BRM-8-1 (0.6%) respectively at 55 DAS. The maximum and minimum AUDPC (A) values were observed in Banka Local Mung-1 (126.2) and BRM-8-1 (3.9) respectively. Among all the 36 genotypes screened, BRM-8-1 (0.6%) was observed to be resistant (R) to yellow mosaic disease (YMD). The three genotypes namely Meha, IPM-409-4, IPM-2-14 were found to be moderately resistant (MR) with disease severities of 9.3%, 6% and 6.7% respectively. The two genotypes namely GP-276 and DMG-1105-1-2 were found to be highly susceptible (HS) with disease severity percent of 56.3 and 53.3 respectively. The results indicated that BRM-8-1 can be used as donor MYMV resistant parent to develop commercial high yielding resistant varieties. GP-276 and DMG-1105-1-2 can also be used as MYMV susceptible varieties in future breeding programmes.