Multiplication of a plant using in vitro culture technique greatly influences with the composition of culture media. Identification of suitable culture media is pre-requisite for getting maximum pace of micropropagation. Therefore, the efficacies of six different culture media for multiplication of Aloe vera were evaluated under in vitro condition using lateral shoot explant. An optimum level of BAP and IBA were used for shoot proliferation and root induction, respectively. Significant variation was obtained in all media types for all the characters studied. MS medium was found best for direct shoot proliferation as it induced highest number of shoot (6.30±0.23) per explant with longest shoot length (3.17±0.09 cm). The order of efficacy of different media for shoot proliferation was as MS medium > Woody plant medium > Nitsch and Nitsch medium > Whites medium > Knudson Solution-C > Schenk and Hildebrandt medium. For root induction, Woody plant medium revealed maximum number of roots (6.10±0.23) per explant, followed by MS medium. The effectiveness of different media for root proliferation were as Woody plant medium > MS medium > Nitsch and Nitsch medium > Whites medium > Knudson Solution-C > Schenk and Hildebrandt medium. Highest survival rate (85 %) during hardening and acclimatization was also obtained in Woody plant medium mediated rootlets. The identified culture media can be recommended for high frequency large scale multiplication of Aloe vera.