The present investigation entitled “Quality studies on twenty genotypes of Mango under Western Odisha condition” was carried out at experimental plot, College of Horticulture, Chiplima, during 2019-20 and 2020-21. The experiment was laid out in Randomized block design with twenty treatments (varieties) and replicated thrice. The mango plants were planted at a spacing of 5 m × 5 m. Observations on different quality parameters of fruits were taken during the experiment period in all the twenty cultivars of mango studied. Different fruiting characters under the study were found statistically significant. Maximum fruit breadth, fruit weight, fruit volume, peel weight and stone weight is obtained in Banganapalli (9.1cm, 310.65g, 315ml, 73.2g, 45.56g) respectively. Highest pulp weight, pulp: stone ratio and pulp percentage was obtained in Mallika (212.93g), Dashehari (7.83) and Arka Aruna (78.16) respectively. The data revealed that highest TSS: acid ratio (62.55), total sugar (11.87%), reducing sugar (7.86%) and protein(1.25mg/100g) content were obtained in Amrapali whereas highest ascorbic acid (34.36 mg/100g) and amino acid (0.92mg/100g) was obtained in Mallika. In respect of other qualitative parameters, Amrapali, Mallika and Dashehari were found to be the best compared to the other varieties. showed better performance during the period of investigation.