Assam Lemon (Citrus limon L.) is considered as one of the most important citrus fruits grown in the north-eastern part of India. In spite of its heavy market demand, recent reports indicated an increasing trend of consumer dissatisfaction associated with inconsistent fruit quality. The present work aimed to improve the fruit quality of Assam Lemon by hormonal regulation. Different concentrations of GA3 (20, 40 and 60ppm) and NAA (10, 20 and 30ppm) were applied at three different growth stages of the plant. Various quality attributes of harvested fruits were analyzed. Chromatographic (HPLC) technique was employed for separation and quantification of oxalic, citric, tartaric, D-malic and ascorbic acids. Fruit juice content and TSS-Acidity ratio were positively influenced by GA3 60ppm. Organic acid content of fruit juice was found to be altered by GA3 and NAA. However, significant differential quality attributes were recorded between GA3 and NAA treated fruits. It can be inferred that the fruit quality of Assam Lemon can be appreciably improved by foliar application of GA3 and NAA.