Journal ID : AMA-21-06-2022-11477
[This article belongs to Volume - 53, Issue - 07]
Total View : 384

Title : Qualitative Phytochemical Screening and Exploration of some in vitro Biological activities of Phytolacca latbenia (MOQ.) H. WALTER from Pakistan

Abstract :

The current study was to evaluate secondary metabolites, curative antimicrobial and antioxidant agents of an ethnobotanically important plant i.e., Phytolacca latbenia (Moq.) H. Walter. Phytochemical screening explored the presence of different secondary metabolites. Ethanol extracts of leaves were most potent against all the testing bacterial strains. Antibacterial activity was determined using agar well diffusion method. The zones formed by ethanol extracts of leaves were, 23.16±0.11mm, 18.05±0.04mm, 25.15±0.11mm and 12.75±0.06mm against Staphylococcus aureus, S. epidermis, Acinetobacter baumannii and Pseudomonas aeruginosa, respectively. The chloroform and ethanol extracts showed bigger zone of inhibition as compared to the standard antibiotic against S. aureus and P. aeruginosa. Likewise ethanol extract of leaves has more inhibition potential against S. epidermidis and A. baumannii. This potential was accompanied by using standard five reported assays. Percentage inhibition of DPPH• of fruits (81.08±0.133%) was greater than leaves (69.86±0.183%). Even the sample plant extracts showed the greater potential of inhibition than synthetic antibiotics. Percentage inhibition of ABTS•+ of leaves (59.26±0.140%) was greater than fruits (58.45±0.246%). The values of FRAP assay and TFC of leaves (0.53±0.014 mM Fe2+ equivalent/g and 27±0.02 mg CE/g) were greater than fruits (0.212±0.028 mM Fe2+ equivalent/g and 14.15±0.024 mg CE/g) whereas TPC of fruit (404±0.005 mg GAE /g) were greater than leaves (72.91±0.059 mg GAE /g).

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