Rajmash (Phaseolus vulgaris L.) is the most famous pulse crop preferred by consumers in Jammu and Kashmir. The pole type varieties are grown in the temperate region as an intercrop with maize. A promising pure line selection of Rajmash from Bhaderwah (Bhaderwah Rajmash 104) was selected on the basis of its quality, yield parameters and resistance to diseases/pests. The pure line selection was characterized using molecular markers viz., Inter Simple Sequence Repeats (ISSR), Randomly Amplified Polymorphic DNA (RAPD) and EST- Simple Sequence Repeats (EST- SSR). DNA polymorphism was shown 10 RAPD primers, 8 ISSR primers and 9 EST-SSR primers.