Journal ID : AMA-15-03-2024-12951
[This article belongs to Volume - 55, Issue - 03]
Total View : 432

Title : Potential Analgesic, Anti-inflammatory and Antioxidant Activities OF hydroethanolic Extract OF Crataegus oxycantha L. Berries

Abstract :

The present study was designed to evaluate the antioxidant, anti-inflammatory and analgesic activities of hydroethanolic ex¬tract from Crataegus oxycantha L. fruit, a plant widely used as natural treatment of cardiovascular diseases in folk medicine. The antioxidant activity was determined by Phosphomolybdate, H2O2 radical scavenging, and β-carotene bleaching assays. The anti-inflammatory was evaluated by croton oil -induced ear edema in mice and analgesic activity was carried out by acetic acid induced writhing in mice. The polyphenolic and flavonoids contents of C. oxycantha extract were found to be 56.5 ± 2.49 mg EGA/g of dry extract and 0.43 ± 0.09 mg EQ/ g of dry extract, respectively. Whereas the tannins content was 48. 30 ± 0. 9 mg ETA/ g of dry extract. C. oxycantha ethanolic extract showed a good ability to scavenge hydrogen peroxide with an IC50 value of 2.695 ± 0.121 ± mg / ml. In addition, this extract exhibited a high total antioxidant capacity (TAC) in molybdate phosphate test (EC50 of 0.145 ± 0.006 mg / ml). The administration of C. oxycantha extract at the dose of 200 and 600 mg/kg caused significant inhibition of inflammation in croton oil-induced ear edema in mice. In acetic acid-induced writhing model, C. oxycantha extract reduced abdominal cramps at a dose of 600 mg/kg with 69.92% of inhibition. The results suggest that the polyphenolic extract of C. oxycantha L. possesses antioxidant, anti-inflammatory and analgesic activities which could be tested as drug candidates against oxidative and inflammation-related pathological processes.

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