This study aimed to evaluate, for the first time, the phenolic contents, antioxidant, antibacterial, and antifungal properties of the Hodna sub-arid lentisk seed oils obtained through hot (traditional method) and cold extraction techniques. The cold pressing process resulted in a much greater yield (15%) compared to the traditional method (5%). Both Hodna lentisk oils obtained by cold pressing (HLCO) and hot extraction (HLHO) were rich in polyphenols, but the total phenolic content of HLCO (94.54 mg GAE/100 g) was twice that of HLHO (48.02 mg GAE/100 g), making HLCO a richer source of phenolic compounds. HLHO and HLCO showed very high free radical scavenging activity, which were respectively 113.81 and 107.47 mM TE/100 g. Limited inhibitory effects were observed against a panel of five pathogenic bacteria and six fungal strains. The sub-arid origin of P. lentiscus oils, more particularly the cold-extracted one, may be a natural source of promising phenolics compounds that could be incorporated into new products or replace synthetic compounds in the health, food, and cosmetics sectors.