Traditional medicine has always held a significant place in Algerian medication traditions. This thesis contributes to the study of the biological activities of Buxus dioica. The preparation of various extracts was followed by phytochemical screening. Subsequently, an evaluation of antioxidant activity (total capacity and DPPH scavenging) and antimicrobial activity (solid medium diffusion) was conducted. Three extracts were prepared: methanolic, ethanolic (macerated), and aqueous (infused) extracts. The results showed that the aqueous extract had the highest yield at 9.3% among the studied extracts. Phytochemical screening revealed the presence of various secondary metabolites (tannins, flavonoids, saponins, and starch). The evaluation of antioxidant activity revealed that this plant has excellent antioxidant properties, with a percentage of 92% and an IC50 of approximately 2.01 mg/ml. Microorganism sensitivity tests showed that Candida albicans ATCC 10231 A14 was the most sensitive to the methanolic extract (22 mm) by the solid medium diffusion method.