The experiment was conducted to evaluate the phytochemicals of Murraya koenigii (L.) Spreng leaves collections from 10 different sites of three districts (Kangra, Hamirpur and Bilaspur) of Himachal Pradesh. The extraction and estimation of collected leaves was carried out in the Laboratory of Department of Forest Products, College of Forestry, Dr. YS Parmar university of Horticulture and Forestry, Nauni, Solan during the year 2022-23. The data recorded were subjected to statistical analysis under Completely Randomized Design (CRD). Analysis of variance was carried out and critical difference at 5 percent level of significance. It was reported that maximum chlorophyll a content was found in the sample collected from site Jhaniari (District Hamirpur) -T10 i.e. (0.88 mg/g), chlorophyll b was found in the sample collected from site T3 Nadaun (Hamirpur) -(0.65 mg/g) and maximum value for total chlorophyll was recorded in the sample collected from site Jhaniari (Hamirpur) - T10 i.e. (1.44 mg/g). Maximum carotenoid content was recorded in samples collected from Dheera (District, Kangra) -T1 i.e. (0.71 mg/g) and minimum was recorded in Jhaniari (District, Hamirpur) – T10 i.e. (0.55 mg/g). Among different sites alkaloids was present and saponin was found to be absent. Maximum phenolic content was found in Jhaniari (district, Hamirpur) -T10 i.e. (417.68 ± 0.92 GAE/g) and minimum was recorded in Dheera (Kangra) -T1 i.e. (291.39 ± 1.33 GAE/g). Maximum flavonoid content was recorded in Jhaniari (Hamirpur) -T10 i.e. (324 ± 0.96RE/g) and minimum was recorded in Dheera (Kangra) -T1 i.e. (204 ± 1.79 RE/g).