A comparative study on Physiological Stage-Specific Liver Enzyme Variations in Jersey Crossbred Cows was done. A total of 72 healthy Jersey crossbred cattle (n=72), including 12 female calves and adult cows aged 3-6 years with a good body condition score (5-6 out of 10) were selected. They were divided into six groups, each group comprised of twelve (n=12) animals, Group 1: female calves, Group 2: heifers, Group 3: pregnant lactating, Group 4: non-pregnant lactating, Group 5: pregnant dry and Group 6: non-pregnant dry Jersey crossbred cows. Serum AST, ALT and LDH were higher in heifers than female calves whereas ALP, GGT and CK were higher in female calves than heifers. Due to increased liver activity and metabolic demands in heifers associated with growth and development. In growing calves, higher ALP levels observed due to active bone formation and skeletal growth. Calves have higher levels of GGT due to liver growth and developing state and increased metabolic activity during growth. High CK levels in calves due to rapid muscle growth and development. The pregnant lactating group had higher AST, ALT ALP and GGT followed by non-pregnant lactating group. This might be due to higher metabolic activity and liver function to support milk production and fetal growth during pregnancy and lactation. However, in nonpregnant lactating cows, without the additional demands of pregnancy, the levels might be slightly lower than in pregnant lactating cows. As compared to heifers, nonpregnant dry group had lower serum AST, ALT, ALP and GGT. During the dry period, cows are not producing milk, but pregnancy still requires significant liver function for supporting fetal development, contributing to elevated AST levels. Serum LDH was higher in pregnant dry G 5 followed by non-pregnant dry G 6. Pregnancy requires significant metabolic support for fetal development, contributing to elevated LDH levels in G 5 than G 6. However, in G 3 and G 4, the demands of both lactation and pregnancy increase LDH levels due to higher metabolic activity and tissue turnover to support milk production and fetal growth. Among all the groups, serum CK was highest in the nonpregnant dry group followed non-pregnant lactating group.