Pseudomonas (P) fluorescens promote plant growth by lowering the endogenous ethylene synthesis in the roots through their 1-aminocylopropane-1-carboxylate-deaminase (ACCD) activity. However, in the environs of the roots may decrease the efficiency of these P. fluorescens by stimulating ACC- oxidase activity resulting in greater ethylene production by the roots. So this study was designed to assess the performance of P. fluorescens containing ACC-deaminase for improving growth and yield of maize. In total, 16 isolates of P. fluorescens obtained from fermentor under different temperature (25, 28, 31, 34° C) and pH (6.7, 7.2, 7.7, 8.2) based on ACCD activity and population kinetics. It was observed that P. fluorescens 06 isolate significantly enhanced the seed emergence by 31.1 and 28.7% at 05 and 09 days after sowing (DAS), respectively, while the isolate of P. fluorescens 10 increased emergence 30.8% at 07 DAS over control. The isolates P. fluorescens 10 significantly increased number and length of roots by 30.0, and 22.5%, respectively at 55 DAS and P. fluorescens 06 isolates significantly increased fresh and dry weight of brace roots by 25.0, and 26.2%, respectively over control. The isolates of P. fluorescens 10 at 75 DAS significantly increased root number, fresh, and dry weight of brace root by 30.2, 28.1, and 25.7%, respectively and isolates P. fluorescens 06 significantly increased root length 22.4% over control. The maximum plant height recorded with isolates P. fluorescens 10 by 29.3, and 29.1% at knee stage and silking stage, respectively and P. fluorescens 06 at tasseling stage by 31.1%, over control. Application of isolates P. fluorescens 06 accounted for highest number of leaves at knee height and silking stage and P. fluorescens 10 at tasseling stage by 32.6, 27.2 and 31.1% over control. Whereas, the application of isolate P. fluorescens 06 yielded 11.8, 13.3 and 21.7% higher total chlorophyll at knee height, tasseling and silking stage, respectively over control. Application of P. fluorescens 06 isolate observed significantly 19.2, 28.0, 24.6, and 26.3% higher fresh and dry weight over control at knee height and silking stage respectively, at tasseling stage the response of P. fluorescens 10 isolate yielded 26.6 and 23.8% higher fresh and dry weight over control. The isolates P. fluorescens 06 significantly increased the grain and stover yield 24.9 and 31.6% of maize over control.