An efficient shoot proliferation response from the anther derived microshoots (1-2 mm) of two heterozygous populations of African marigold (Desi Orange and Desi Yellow) to various invitro media compositions was investigated. The best multiple shoot induction (about 3.34 shoots/explant and 11.57 shoots/explant) after 30 and 60 days of culturing respectively and minimum number of days taken for shoot proliferation (4.05 days) was observed after the microshoot explants were cultured on solid Murashige and Skoog medium (MS) supplemented with NAA (0.1 mgL-1) + meta topolin (0.5 mgL-1). 100 percent shoot proliferation was recorded in the MS medium devoid of any growth regulators and NAA (0.1 mgL-1) + meta topolin (0.5 mgL-1). Length of the shoots was also found maximum (4.14 cm) in the MS medium supplemented with NAA (0.1 mgL-1) + meta topolin (0.5 mgL-1). Between the two genotypes, Desi Orange showed better response in terms of shoot proliferation percentage (61.44%), days for shoot proliferation (8.52 days), average number of shoots per microshoots (2.16 and 6.48 shoots/explant) after 30 and 60 days of culturing and length of the shoots (2.71cm) than Desi Yellow. Maximum rooting (95.89%) and roots/shoot (38.11) were obtained with ½ MS + IBA (0.5 mgL-1). Also, Desi Orange had maximum rooting (91.40%) and roots/shoot (30.84). Meta Topolin and IBA were found superior in increasing the shoot and root growth respectively.