An in-vitro protocol was developed for rapid mass multiplication of rose cv. ‘Rose Sherbet’ under in-vitro condition using axillary buds as explants segment. Out of different pre-treatments for explants, the highest explant survival (76.41 %) and less microbial contamination (13.5) was obtained with treatment (T1) 0.2% Carbendazim + 200 mg/L 8-HQC for 3 hours. Ex plant surface sterilized with 0.1 % HgCl2 for 5 minutes exhibited the maximum survival percentage (74.46%). MS medium supplemented with 5.0 mg/L BAP + 0.1 mg/L NAA + 0.5 mg/L GA3 was found most effective for culture establishment, however, MS medium comprising 4.0 mg/L BAP + 0.1mg/L NAA+ 0.2 mg/L kinetin along with 40 mg/L adenine sulphate was found to be better for shoot proliferation with highest number of micro shoots (4.96 shoots/explant). Elongation of plantlets and maximum internode length was recorded in MS + GA3 2.0 mg/L while rooting of micro shoots was induced on MS basal medium supplemented with IBA (0.5 mg/L). The regenerated plantlets were efficiently hardened in plastic pots (4”) filled with coco peat + vermiculite + perlite (1:1:1) which is further moistened with half strength MS medium salts and covered with polythene. Survival percentage, plant height, number of branches and number of leaves/plants were found maximum in this treatment. Thereafter, plants were successfully transferred to the glasshouse with good survival.