The current study investigated an effective way to extract C-phycocyanin and evaluate its antioxidant potential from the sub-aerial cyanobacteria. Two cyanobacterial species were isolated and identified as Oculatella crustae-formantes and Desertifilum dzianensis in order to standardise and characterize the Phycocyanin production from subaerial cyanobacteria and its antioxidant Potential. The specific growth rate and doubling time of the isolates were calculated. Sodium phosphate buffer (pH 7, 50mM) was found to be the most efficient buffer for C-phycocyanin yield. Further, the BG-11 medium under LED fluorescent red tube light was discovered to be the best optimization condition for enhancing C-phycocyanin synthesis from the isolates. Spectrophotometry technique and Fourier Infra red technique were utilised for the C-PC characterization. Additionally, the purified C-phycocyanin was evaluated for antioxidant activities. In both cases, the IC50value of C-PC is found to be higher than the standards used. This indicates the significant potential of sub-aerial cyanobacteria to produce highly purified phycocyanin with notable antioxidant properties, suggesting potential applications in the food and pharmaceutical industries.