This study has the objective to standardize the optimal nutritional and physical parameters associated with the growth and sporulation of fungal biological control agent named C. rosea. Among the six isolates of biocontrol agent, TNAU CR 01 was used in the present study. The effects of different substrates amended on nutritional medium were tested for the growth of fungal mycelium by petri plate method. Different temperature range was maintained to found the optimal environmental set up for improving the growth in BOD incubator. The pH range from acidic to alkaline was tested for their role in increasing the biomass and conidial production of C. rosea under liquid cultural condition. Among the six isolates of biocontrol agent, TNAU CR 01 was used in the present study. The effect of different substrates amended on nutritional medium was tested for the growth of fungal mycelium by petri plate method. Different temperature range was maintained to found the optimal environmental set up for improving the growth in BOD incubator. The pH range from acidic to alkaline was tested for their role in increasing the biomass and conidial production of C. rosea under liquid cultural condition.