Experiments were conducted in two locations under the acidic soil conditions of Nagaland. Growth, yield, yield attributes and quality of soybean were recorded, analysed and computed statistically during 2019, 2020 and pooled respectively. It was observed that the parameters such as plant height, no. of nodules plant-1, fresh weight of nodules, dry weight of nodules, seeds pod-1, pods plant-1, seed and stover yield, protein content and yield, oil content and yield and soil fertility after harvest were significantly increased @ 5 kg ha-1 ZnSO4 H2O + RDF during both the experimental years as well as in pooled respectively. The fatty acids composition in soybean such as palmitic, stearic, oleic, linoleic and linolenic acid were also found to be significantly increased @ 5 kg ha-1 ZnSO4 H2O + RDF among the various Zn sources applied. From the experiment conducted, it was observed that soybean responded well to application of Zinc sources in balanced doses.