Feeding practices and nutritional status data of lactating cattle were collected from livestock farm complex, ANDUA&T, Kumarganj Ayodhya and unorganized farms of Middle Gangetic Plains of Uttar Pradesh, India. A total of 100 cattle were selected as per stratified randomized design (SRD) from organized and unorganized farm. Nutritional status of lactating cattle was ascertained through body weight, milk yield and feed intake data as well as from serum metabolic profile values. Cattle of organized farm were heavier (P<0.05) than unorganized farm. The milk yield of cattle were significantly (P<0.05) higher in organized farm than unorganized farm. In organized farm cattle were mainly maintained on wheat straw, berseem, sorghum and concentrate while in unorganized farm they were maintained on wheat straw and available green fodder and/or local grasses along with very low amount of homemade concentrates. Lactating cattle of unorganized farms were deficient in DM intake and the deficiency of CP and TDN was significantly (P<0.05) higher in organized farm than unorganized farm. Among blood biochemical parameters, the serum glucose, total protein, albumin and globulin were found within the normal range and did not vary significantly (P>0.05) between farm. Thus, it is concluded that the dairy animals in Middle Gangetic Plain were fed with traditional manner and they were deficient in DM, CP and TDN supply.