Crossbred cows' milk fat percentage data were collected from the Bharathi Agro-Industrial Foundation, Pune. The phenotypic data collected were from smallholder dairy systems from six states from 2016 and 2022. The data were subject to quality control and R statistical software was used to find the significance among the non-genetic factors affecting the milk fat percentage. The overall mean milk fat percentage was 3.83±0.81 among the crossbred cows. The mean values for different years ranged between 3.47±0.24 and 3.92±0.88, whereas the mean values for the three seasons were 3.82±0.79, 3.82±0.82 and 3.86±0.82 respectively. The later stage of lactation had a higher mean of 3.98±0.78 as compared with 3.57±0.82 during early lactation. A significant difference (P<0.001) was noticed among all the non-genetic factors considered for the present study. The results will be useful to study the performance of crossbred under different environmental conditions.