Nano-fertilizer plays an important role in improving the fertilizer use efficiency owing to their unique properties like smaller size, high specific surface area; high surface energy and high solubility in the large nutrient requirement staple food crops like wheat (Triticum aestivum L.) to meet the global food security by reducing the drastic hazards of excessive use of fertilizers. This experiment was conducted for two years 2020-21; 2021-22 of Rabi season with the aim of this study was to evaluate the effect of nano-fertilizer mediated nutrients along with the conventional fertilizers and biofertilizers on crop growth and yield of wheat. The treatments assigned in this study are fourteen viz. Control (No fertilizer) T1, 100%RDF (NPK-150:75:60 kg/ha)T2, 100 % NPK + Nano Zn Spray (After I irrigation)T3, 100 % NPK + Bio-stimulant Spray (After I irrigation)T4, 100% NPK + seed treatment with NPK consortiaT5, 100% NPK + seed treatment with AzotobacterT6, 75 % NPK + NPK Consortia @ 250ml in 3 lit water 60 kg-1T7, 75 % NPK + NPK spray @ 15gm per lit (After 1st irrigation)T8, 75 % NPK + NPK Consortia + Nano N spray (After 1st irrigation)T9, 75 % NPK + NPK Consortia + NPK spray (After 1st irrigation )T10,75 % NPK + NPK Consortia + NPK spray (After 1st irrigation) +Bio-stimulant Spray (After 1st irrigation)T11, 75 % NPK + NPK Consortia + NPK spray (After 1st irrigation) + Bio-stimulant Spray (After 1st irrigation) + Nano Zn spray (After 1st irrigation)T12, 75 % NPK + NPK Consortia + Nano Zn spray (After 1st irrigation)T13, 75 % NPK + NPK Consortia +Nano N spray (After 1st irrigation) + Nano Zn spray 1st irrigationT14. The treatment T3 receiving 100 % NPK + Nano Zn Spray (After I irrigation)) was observed significantly higherin yield parameters, quality parameters, nutrient use efficiency, soil microbial properties and profitability of both the years compared to other treatments and which is at par with T12 (75 % NPK + NPK Consortia + NPK spray (After 1st irrigation) + Bio-stimulant Spray (After 1st irrigation) + Nano Zn spray (After 1st irrigation)). T3 recorded44.75, 88.00, 100.8 and 179.80 percent yield, protein content, gross returns and net returns higher than T1 control with no nutrient application and 11.15, 22.44, 11.16 and 12.05 percent yield, protein content, gross returns and net returns higher over T2 only RDF application respectively.