A field experiment was conducted with 114 germplasm to study the morphological characterization using self assumed description and nominal variables of morphological characters, which were used as an input for Nbclust hierarchical cluster analysis in which clustering was done using Ward’s minimum variance method and Euclidean’s method of genetic distance was derived. In this study, niger breeding lines were grouped into six clusters based on analysis of divergence at the genetic distance. They revealed considerable amount of genetic diversity. The largest cluster among all is cluster II. Cluster II has 34 germplasm, cluster I has 29, Cluster III has 24, cluster V contain 13, cluster IV has 9 and cluster VI has 5 germplasm. Whereas, morphological characterization observation were recorded on 12 traits viz., leaf color, leaf shape, leaf serration of margin, leaf angle of branching, stem hairiness, stem color, color of ray florets, plant branching habit, pollen color, seed color, seed shape and seed texture, etc.