Fowl cholera is an acute, fatal septicemic disease of various domestic and wild bird species, which is responsible for significant loss in poultry husbandry. The present study was carried out to find out the cause for mortality in quail chicks in Thanjavur district of Tamilnadu, India. The farmer reported that out of 2000 quail chicks in the broiler farm 213 nine day old quail chicks were died suddenly with a history of dullness, depression and mucous discharge from the mouth. Based on the report 20 heart swabs were collected from the dead birds. The targeted bacteria from the samples were isolated, identified and characterized based on their morphology, staining, cultural and biochemical characters. The organisms were gram negative and appeared singly or pairs in Gram staining, whereas in Leishman’s stain, bipolar shaped organisms were observed. All tested samples were found positive for Pasteurella multocida. Three suspected cultures were subjected to molecular characterization using the Pasteurella multocida species specific primers for KMT1 gene found to be positive for Pasteurella multocida. In type specific PCR reaction, using specific primer which gave positive results for 511 bp gene fragment of capE gene were confirmed as P. multocida Type A. Study shows P. multocida type A is prevalent in quail chicks hence care must be taken to control of the disease.